bulk rna sequencing data  (GraphPad Software Inc)

Journal: Clinical & Translational Immunology

Article Title: The contribution of the CRP/CD64 axis to renal cancer progression by inducing protumor activation of tumor‐associated macrophages

doi: 10.1002/cti2.70013

Figure Lengend Snippet: CRP induces the production of IL‐6 via the CRP/CD64 signalling pathway in macrophages. (a) The IL‐6 secretion from HMDMs stimulated with CRP (20 μg mL −1 ) during incubation with anti‐CD16, CD32, CD64 and IL‐6R neutralising antibodies for 24 h was measured by ELISA ( n = 4). Experiments were repeated three times with almost identical results. The Kruskal–Wallis test was performed. * P < 0.01, ** P < 0.001. (b) The phosphorylated STAT3 expression in HMDMs after treatment with CRP (20 μg mL −1 ) during incubation with anti‐CD64 and IL‐6R neutralising antibodies for 24 h was measured by western blotting. Experiments were repeated three times with almost identical results. (c) The phosphorylated STAT3 expression in ccRCC cell lines (786‐O and MAMIYA) after treatment with MCM or CRP‐MCM during incubation with anti‐CD64 neutralising antibody (5 μg mL −1 ) for 24 h was measured by western blotting. Experiments were repeated three times with almost identical results. (d) The volcano plots of the gene expression in CRP‐treated HMDMs as measured by RNA‐seq.

Article Snippet: Bulk RNA sequencing data of 528 ccRCC (KIRC) cases from TCGA are summarised in The Human Protein Atlas ( https://www.proteinatlas.org/about/download ).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Gene Expression, RNA Sequencing